Modern bioanalysis of proteins by electrophoretic techniques.

نویسندگان

  • Sona Krizkova
  • Marketa Ryvolova
  • Michal Masarik
  • Ondrej Zitka
  • Vojtech Adam
  • Jaromir Hubalek
  • Tomas Eckschlager
  • Rene Kizek
چکیده

In 1957, protein rich in cysteine able to bind cadmium was isolated from horse kidney and named as metallothionein according to its structural properties. Further, this protein and metallothionein-like proteins have been found in tissues of other animal species, yeasts, fungi and plants. MT is as a potential cancer marker in the focus of interest, and its properties, functions, and behavior under various conditions are intensively studied. Our protocol describes separation of two major mammalian isoforms of MT (MT-1 and MT-2) using capillary electrophoresis (CE) coupled with UV detector. This protocol enables separation of MT isoforms and studying of their basic behavior as well as their quantification with detection limit in units of ng per μL. Sodium borate buffer (20 mM, pH 9.5) was optimized as a background electrolyte, and the separation was carried out in fused silica capillary with internal diameter of 75 μm and electric field intensity of 350 V/cm. Optimal detection wavelength was 254 nm.

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عنوان ژورنال:
  • Methods in molecular biology

دوره 1129  شماره 

صفحات  -

تاریخ انتشار 2014